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Quantum Dot Inc quantum dot-based immunohistofluorescence
H460 lung cancer xenografts were treated with increasing doses of BDA-366 for 14 days (n = 6 mice per group, 3 sections per animal). Bcl2 and Bax conformation changes were analyzed in tumor tissues at the end of experiments by <t>QD-IHF</t> using anti-Bcl2/BH3 domain antibody or anti-6A7 Bax antibody, respectively, and quantified as described in “Supplemental Experimental Procedures”. Scale bar represents 10 um. Error bars represent ± S.D. See also Figure S5.
Quantum Dot Based Immunohistofluorescence, supplied by Quantum Dot Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantum+dot-based+immunohistofluorescence/quantum+dot+based+immunohistofluorescence/pmc04470473-400-2-0
Average 90 stars, based on 1 article reviews
quantum dot-based immunohistofluorescence - by Bioz Stars, 2026-09
90/100 stars

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1) Product Images from "Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy"

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy

Journal: Cancer cell

doi: 10.1016/j.ccell.2015.04.010

H460 lung cancer xenografts were treated with increasing doses of BDA-366 for 14 days (n = 6 mice per group, 3 sections per animal). Bcl2 and Bax conformation changes were analyzed in tumor tissues at the end of experiments by QD-IHF using anti-Bcl2/BH3 domain antibody or anti-6A7 Bax antibody, respectively, and quantified as described in “Supplemental Experimental Procedures”. Scale bar represents 10 um. Error bars represent ± S.D. See also Figure S5.
Figure Legend Snippet: H460 lung cancer xenografts were treated with increasing doses of BDA-366 for 14 days (n = 6 mice per group, 3 sections per animal). Bcl2 and Bax conformation changes were analyzed in tumor tissues at the end of experiments by QD-IHF using anti-Bcl2/BH3 domain antibody or anti-6A7 Bax antibody, respectively, and quantified as described in “Supplemental Experimental Procedures”. Scale bar represents 10 um. Error bars represent ± S.D. See also Figure S5.

Techniques Used:

Related Articles

Immunohistofluorescence:

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy
Article Snippet: BDA-366 induces Bcl2 conformational change in tumor tissues Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy
Article Snippet: Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Article Title: Tapping the potential of quantum dots for personalized oncology: current status and future perspectives.
Article Snippet: Cancer is one of the most serious health threats worldwide, with an estimated 12.7 million new cases and 7.6 million cancer deaths in 2008 according to the GLOBOCAN estimates [1].. Although increasingly more cancer patients could be diagnosed and treated early due to the progress in cancer screening and control programs [1,2], the survival of most patients remains poor with no significant reduction in mortality over the past several decades [3–6], especially in develop ing countries [4,7].. It has long been recognized that cancer is not merely a local problem, but a complex systemic disease.

Article Title: Small Molecule Bax Agonists for Cancer Therapy
Article Snippet: Quantum Dot-based immunohistofluorescence (QD-IHF) and quantification.

Article Title: Niclosamide Overcomes Acquired Resistance to Erlotinib through Suppression of STAT3 in Non–Small Cell Lung Cancer
Article Snippet: Quantum dot-based immunohistofluorescence analysis of pEGFR and pSTAT3 in tumor tissues The QD-IHF technique is a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide (33–35, 40).

Article Title: Niclosamide Overcomes Acquired Resistance to Erlotinib through Suppression of STAT3 in Non–Small Cell Lung Cancer
Article Snippet: Quantum dot-based immunohistofluorescence and its signal quantification QD-IHF for measurement of pSTAT3 and pEGFR in tumor tissueswas conducted as described previously (33– 35).

Immunostaining:

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy
Article Snippet: BDA-366 induces Bcl2 conformational change in tumor tissues Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy
Article Snippet: Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Article Title: Tapping the potential of quantum dots for personalized oncology: current status and future perspectives.
Article Snippet: Cancer is one of the most serious health threats worldwide, with an estimated 12.7 million new cases and 7.6 million cancer deaths in 2008 according to the GLOBOCAN estimates [1].. Although increasingly more cancer patients could be diagnosed and treated early due to the progress in cancer screening and control programs [1,2], the survival of most patients remains poor with no significant reduction in mortality over the past several decades [3–6], especially in develop ing countries [4,7].. It has long been recognized that cancer is not merely a local problem, but a complex systemic disease.

Article Title: Small Molecule Bax Agonists for Cancer Therapy
Article Snippet: Quantum Dot-based immunohistofluorescence (QD-IHF) and quantification.

Article Title: Niclosamide Overcomes Acquired Resistance to Erlotinib through Suppression of STAT3 in Non–Small Cell Lung Cancer
Article Snippet: Quantum dot-based immunohistofluorescence analysis of pEGFR and pSTAT3 in tumor tissues The QD-IHF technique is a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide (33–35, 40).

Article Title: Niclosamide Overcomes Acquired Resistance to Erlotinib through Suppression of STAT3 in Non–Small Cell Lung Cancer
Article Snippet: Quantum dot-based immunohistofluorescence and its signal quantification QD-IHF for measurement of pSTAT3 and pEGFR in tumor tissueswas conducted as described previously (33– 35).

Formalin-fixed Paraffin-Embedded:

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy
Article Snippet: BDA-366 induces Bcl2 conformational change in tumor tissues Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy
Article Snippet: Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Article Title: Tapping the potential of quantum dots for personalized oncology: current status and future perspectives.
Article Snippet: Cancer is one of the most serious health threats worldwide, with an estimated 12.7 million new cases and 7.6 million cancer deaths in 2008 according to the GLOBOCAN estimates [1].. Although increasingly more cancer patients could be diagnosed and treated early due to the progress in cancer screening and control programs [1,2], the survival of most patients remains poor with no significant reduction in mortality over the past several decades [3–6], especially in develop ing countries [4,7].. It has long been recognized that cancer is not merely a local problem, but a complex systemic disease.

Article Title: Small Molecule Bax Agonists for Cancer Therapy
Article Snippet: Quantum Dot-based immunohistofluorescence (QD-IHF) and quantification.

Article Title: Niclosamide Overcomes Acquired Resistance to Erlotinib through Suppression of STAT3 in Non–Small Cell Lung Cancer
Article Snippet: Quantum dot-based immunohistofluorescence analysis of pEGFR and pSTAT3 in tumor tissues The QD-IHF technique is a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide (33–35, 40).

Article Title: Niclosamide Overcomes Acquired Resistance to Erlotinib through Suppression of STAT3 in Non–Small Cell Lung Cancer
Article Snippet: Quantum dot-based immunohistofluorescence and its signal quantification QD-IHF for measurement of pSTAT3 and pEGFR in tumor tissueswas conducted as described previously (33– 35).

Immunohistochemistry:

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy
Article Snippet: BDA-366 induces Bcl2 conformational change in tumor tissues Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy
Article Snippet: Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Article Title: Tapping the potential of quantum dots for personalized oncology: current status and future perspectives.
Article Snippet: Cancer is one of the most serious health threats worldwide, with an estimated 12.7 million new cases and 7.6 million cancer deaths in 2008 according to the GLOBOCAN estimates [1].. Although increasingly more cancer patients could be diagnosed and treated early due to the progress in cancer screening and control programs [1,2], the survival of most patients remains poor with no significant reduction in mortality over the past several decades [3–6], especially in develop ing countries [4,7].. It has long been recognized that cancer is not merely a local problem, but a complex systemic disease.

Article Title: Small Molecule Bax Agonists for Cancer Therapy
Article Snippet: Quantum Dot-based immunohistofluorescence (QD-IHF) and quantification.

Article Title: Niclosamide Overcomes Acquired Resistance to Erlotinib through Suppression of STAT3 in Non–Small Cell Lung Cancer
Article Snippet: Quantum dot-based immunohistofluorescence analysis of pEGFR and pSTAT3 in tumor tissues The QD-IHF technique is a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide (33–35, 40).

Article Title: Niclosamide Overcomes Acquired Resistance to Erlotinib through Suppression of STAT3 in Non–Small Cell Lung Cancer
Article Snippet: Quantum dot-based immunohistofluorescence and its signal quantification QD-IHF for measurement of pSTAT3 and pEGFR in tumor tissueswas conducted as described previously (33– 35).

In Situ:

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy
Article Snippet: BDA-366 induces Bcl2 conformational change in tumor tissues Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy
Article Snippet: Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Article Title: Tapping the potential of quantum dots for personalized oncology: current status and future perspectives.
Article Snippet: Cancer is one of the most serious health threats worldwide, with an estimated 12.7 million new cases and 7.6 million cancer deaths in 2008 according to the GLOBOCAN estimates [1].. Although increasingly more cancer patients could be diagnosed and treated early due to the progress in cancer screening and control programs [1,2], the survival of most patients remains poor with no significant reduction in mortality over the past several decades [3–6], especially in develop ing countries [4,7].. It has long been recognized that cancer is not merely a local problem, but a complex systemic disease.

Article Title: Small Molecule Bax Agonists for Cancer Therapy
Article Snippet: Quantum Dot-based immunohistofluorescence (QD-IHF) and quantification.

Article Title: Niclosamide Overcomes Acquired Resistance to Erlotinib through Suppression of STAT3 in Non–Small Cell Lung Cancer
Article Snippet: Quantum dot-based immunohistofluorescence analysis of pEGFR and pSTAT3 in tumor tissues The QD-IHF technique is a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide (33–35, 40).

Article Title: Niclosamide Overcomes Acquired Resistance to Erlotinib through Suppression of STAT3 in Non–Small Cell Lung Cancer
Article Snippet: Quantum dot-based immunohistofluorescence and its signal quantification QD-IHF for measurement of pSTAT3 and pEGFR in tumor tissueswas conducted as described previously (33– 35).

Staining:

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy
Article Snippet: BDA-366 induces Bcl2 conformational change in tumor tissues Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy
Article Snippet: Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Article Title: Tapping the potential of quantum dots for personalized oncology: current status and future perspectives.
Article Snippet: Cancer is one of the most serious health threats worldwide, with an estimated 12.7 million new cases and 7.6 million cancer deaths in 2008 according to the GLOBOCAN estimates [1].. Although increasingly more cancer patients could be diagnosed and treated early due to the progress in cancer screening and control programs [1,2], the survival of most patients remains poor with no significant reduction in mortality over the past several decades [3–6], especially in develop ing countries [4,7].. It has long been recognized that cancer is not merely a local problem, but a complex systemic disease.

Article Title: Small Molecule Bax Agonists for Cancer Therapy
Article Snippet: Quantum Dot-based immunohistofluorescence (QD-IHF) and quantification.

Article Title: Niclosamide Overcomes Acquired Resistance to Erlotinib through Suppression of STAT3 in Non–Small Cell Lung Cancer
Article Snippet: Quantum dot-based immunohistofluorescence analysis of pEGFR and pSTAT3 in tumor tissues The QD-IHF technique is a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide (33–35, 40).

Article Title: Niclosamide Overcomes Acquired Resistance to Erlotinib through Suppression of STAT3 in Non–Small Cell Lung Cancer
Article Snippet: Quantum dot-based immunohistofluorescence and its signal quantification QD-IHF for measurement of pSTAT3 and pEGFR in tumor tissueswas conducted as described previously (33– 35).

Amplification:

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy
Article Snippet: BDA-366 induces Bcl2 conformational change in tumor tissues Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy
Article Snippet: Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Article Title: Tapping the potential of quantum dots for personalized oncology: current status and future perspectives.
Article Snippet: Cancer is one of the most serious health threats worldwide, with an estimated 12.7 million new cases and 7.6 million cancer deaths in 2008 according to the GLOBOCAN estimates [1].. Although increasingly more cancer patients could be diagnosed and treated early due to the progress in cancer screening and control programs [1,2], the survival of most patients remains poor with no significant reduction in mortality over the past several decades [3–6], especially in develop ing countries [4,7].. It has long been recognized that cancer is not merely a local problem, but a complex systemic disease.

Article Title: Small Molecule Bax Agonists for Cancer Therapy
Article Snippet: Quantum Dot-based immunohistofluorescence (QD-IHF) and quantification.

Article Title: Niclosamide Overcomes Acquired Resistance to Erlotinib through Suppression of STAT3 in Non–Small Cell Lung Cancer
Article Snippet: Quantum dot-based immunohistofluorescence analysis of pEGFR and pSTAT3 in tumor tissues The QD-IHF technique is a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide (33–35, 40).

Article Title: Niclosamide Overcomes Acquired Resistance to Erlotinib through Suppression of STAT3 in Non–Small Cell Lung Cancer
Article Snippet: Quantum dot-based immunohistofluorescence and its signal quantification QD-IHF for measurement of pSTAT3 and pEGFR in tumor tissueswas conducted as described previously (33– 35).

Fluorescence In Situ Hybridization:

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy
Article Snippet: BDA-366 induces Bcl2 conformational change in tumor tissues Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy
Article Snippet: Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Article Title: Tapping the potential of quantum dots for personalized oncology: current status and future perspectives.
Article Snippet: Cancer is one of the most serious health threats worldwide, with an estimated 12.7 million new cases and 7.6 million cancer deaths in 2008 according to the GLOBOCAN estimates [1].. Although increasingly more cancer patients could be diagnosed and treated early due to the progress in cancer screening and control programs [1,2], the survival of most patients remains poor with no significant reduction in mortality over the past several decades [3–6], especially in develop ing countries [4,7].. It has long been recognized that cancer is not merely a local problem, but a complex systemic disease.

Article Title: Small Molecule Bax Agonists for Cancer Therapy
Article Snippet: Quantum Dot-based immunohistofluorescence (QD-IHF) and quantification.

Article Title: Niclosamide Overcomes Acquired Resistance to Erlotinib through Suppression of STAT3 in Non–Small Cell Lung Cancer
Article Snippet: Quantum dot-based immunohistofluorescence analysis of pEGFR and pSTAT3 in tumor tissues The QD-IHF technique is a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide (33–35, 40).

Article Title: Niclosamide Overcomes Acquired Resistance to Erlotinib through Suppression of STAT3 in Non–Small Cell Lung Cancer
Article Snippet: Quantum dot-based immunohistofluorescence and its signal quantification QD-IHF for measurement of pSTAT3 and pEGFR in tumor tissueswas conducted as described previously (33– 35).

Imaging:

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy
Article Snippet: BDA-366 induces Bcl2 conformational change in tumor tissues Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy
Article Snippet: Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Article Title: Tapping the potential of quantum dots for personalized oncology: current status and future perspectives.
Article Snippet: Cancer is one of the most serious health threats worldwide, with an estimated 12.7 million new cases and 7.6 million cancer deaths in 2008 according to the GLOBOCAN estimates [1].. Although increasingly more cancer patients could be diagnosed and treated early due to the progress in cancer screening and control programs [1,2], the survival of most patients remains poor with no significant reduction in mortality over the past several decades [3–6], especially in develop ing countries [4,7].. It has long been recognized that cancer is not merely a local problem, but a complex systemic disease.

Article Title: Small Molecule Bax Agonists for Cancer Therapy
Article Snippet: Quantum Dot-based immunohistofluorescence (QD-IHF) and quantification.

Article Title: Niclosamide Overcomes Acquired Resistance to Erlotinib through Suppression of STAT3 in Non–Small Cell Lung Cancer
Article Snippet: Quantum dot-based immunohistofluorescence analysis of pEGFR and pSTAT3 in tumor tissues The QD-IHF technique is a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide (33–35, 40).

Article Title: Niclosamide Overcomes Acquired Resistance to Erlotinib through Suppression of STAT3 in Non–Small Cell Lung Cancer
Article Snippet: Quantum dot-based immunohistofluorescence and its signal quantification QD-IHF for measurement of pSTAT3 and pEGFR in tumor tissueswas conducted as described previously (33– 35).



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H460 lung cancer xenografts were treated with increasing doses of BDA-366 for 14 days (n = 6 mice per group, 3 sections per animal). Bcl2 and Bax conformation changes were analyzed in tumor tissues at the end of experiments by <t>QD-IHF</t> using anti-Bcl2/BH3 domain antibody or anti-6A7 Bax antibody, respectively, and quantified as described in “Supplemental Experimental Procedures”. Scale bar represents 10 um. Error bars represent ± S.D. See also Figure S5.
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H460 lung cancer xenografts were treated with increasing doses of BDA-366 for 14 days (n = 6 mice per group, 3 sections per animal). Bcl2 and Bax conformation changes were analyzed in tumor tissues at the end of experiments by <t>QD-IHF</t> using anti-Bcl2/BH3 domain antibody or anti-6A7 Bax antibody, respectively, and quantified as described in “Supplemental Experimental Procedures”. Scale bar represents 10 um. Error bars represent ± S.D. See also Figure S5.
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Image Search Results


H460 lung cancer xenografts were treated with increasing doses of BDA-366 for 14 days (n = 6 mice per group, 3 sections per animal). Bcl2 and Bax conformation changes were analyzed in tumor tissues at the end of experiments by QD-IHF using anti-Bcl2/BH3 domain antibody or anti-6A7 Bax antibody, respectively, and quantified as described in “Supplemental Experimental Procedures”. Scale bar represents 10 um. Error bars represent ± S.D. See also Figure S5.

Journal: Cancer cell

Article Title: Small Molecule Bcl2 BH4 Antagonist for Lung Cancer Therapy

doi: 10.1016/j.ccell.2015.04.010

Figure Lengend Snippet: H460 lung cancer xenografts were treated with increasing doses of BDA-366 for 14 days (n = 6 mice per group, 3 sections per animal). Bcl2 and Bax conformation changes were analyzed in tumor tissues at the end of experiments by QD-IHF using anti-Bcl2/BH3 domain antibody or anti-6A7 Bax antibody, respectively, and quantified as described in “Supplemental Experimental Procedures”. Scale bar represents 10 um. Error bars represent ± S.D. See also Figure S5.

Article Snippet: Quantum dot-based immunohistofluorescence (QD-IHF) technology has recently been developed as a valuable tool for simultaneous and concurrent immunostaining of multiple biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, thereby allowing for quantification of several biomarkers simultaneously on the same tissue slide ( Li et al., 2013 ).

Techniques: